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Artikelnummer: E-EL-0040_96T-ES
3-NT (3-Nitrotyrosine) ELISA Kit, 96T
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 3-NT. During the reaction, Universal 3-NT in the sample or standard competes with a fixed amount of Universal 3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 3-NT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 3-NT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
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Produktspezifikation
Grundlegende Informationen
Marken: Elabscience
Versand: ice bag
Speicher: 2-8℃/-20℃
Haltbarkeit: 12 months
Herkunftsort: China
Technical Specifications
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Sensitivity:0.94 ng/mL
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Shelf Life:12 months
Storage:2-8℃/-20℃
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Parameterinformationen
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Erkennungsbereich: 1.56-100 ng/mL
Testprinzip: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 3-NT. During the reaction, Universal 3-NT in the sample or standard competes with a fixed amount of Universal 3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 3-NT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 3-NT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
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Testzeit: 2 h 30 min
Sensibilität: 0.94 ng/mL
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Wiederherstellung: 80%-120%