Caspase-4 Monoclonal Antibody(Capture), 25 μg

Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (lPS)-induced pyroptosis .Also indirectly activates the NlRP3 and NlRP6 inflammasomes. Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS,GSDMD and Il18 . Effector of the non-canonical inflammasome independently of NlRP3 inflammasome and CASP1: the non-canonical inflammasome promotes pyroptosis through GSDMD cleavage without involving secretion of cytokine Il1B and Il18 . In the non-canonical inflammasome,CASP4 is activated by direct binding to lPS without the need of an upstream sensor . lPS-binding promotes CASP4 activation and CASP4-mediated cleavage of GSDMD,followed by pyroptosis of infected cells and their extrusion into the gut lumen . Also indirectly promotes secretion of mature cytokines (Il1A,Il18 and HMGB1) downstream of GSDMD-mediated pyroptosis via activation of the NlRP3 and NlRP6 inflammasomes . Involved in NlRP3-dependent CASP1 activation and Il1B and Il18 secretion in response to non-canonical activators,such as UVB radiation or cholera enterotoxin . Involved in NlRP6 inflammasome-dependent activation in response to lipoteichoic acid (lTA),a cell-wall component of Gram-positive bacteria,which leads to CASP1 activation and Il1B and Il18 secretion . Involved in lPS-induced Il6 secretion,this activity may not require caspase enzymatic activity. The non-canonical inflammasome is required for innate immunity to cytosolic,but not vacuolar,bacteria. Plays a crucial role in the restriction of S.typhimurium replication in colonic epithelial cells during infection.