Cell Cycle and Apoptosis Analysis Kit, 50 T

Marken: Beyotime

Versand: Blue Ice/Dry Ice

Speicher: -20℃

Haltbarkeit: 12 months

Herkunftsort: China

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Shelf Life:12 months

Storage:-20℃

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Testprinzip: After PI staining, If the fluorescence intensity of cells in G0/G1 phase is consider to be 1, the theoretical value of the fluorescence intensity of G2/M cells containing two copies of genomic DNA is 2 and the fluorescence intensity of S-phase cells undergoing DNA replication is between 1-2. Due to the condensation of nucleus and DNA fragmentation in apoptotic cells, some genomic DNA fragments are lost during the PI staining. Therefore, apoptotic cells exhibit weak PI staining with a fluorescence intensity less than 1, and a sub-G1 peak, also named as apoptotic cell peak, appears when examined by flow cytometry.

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Komponente: Staining Buffer, Propidium Iodide Staining Solution (20X), RNase A (50X)

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Beispiel: 488nm

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Erkennungsinstrument: flow cytometer

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