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Artikelnummer: E-EL-0016_48T-ES
DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit, 48T
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal DHVD3. During the reaction, Universal DHVD3 in the sample or standard competes with a fixed amount of Universal DHVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DHVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal DHVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
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Produktspezifikation
Grundlegende Informationen
Marken: Elabscience
Versand: ice bag
Speicher: 2-8℃/-20℃
Haltbarkeit: 12 months
Herkunftsort: China
Technical Specifications
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Sensitivity:4.69 pg/mL
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Shelf Life:12 months
Storage:2-8℃/-20℃
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Parameterinformationen
Materialien:
Erkennungsbereich: 7.81-500 pg/mL
Testprinzip: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal DHVD3. During the reaction, Universal DHVD3 in the sample or standard competes with a fixed amount of Universal DHVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DHVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal DHVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
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Testzeit: 2 h 30 min
Sensibilität: 4.69 pg/mL
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Wiederherstellung: 80%-120%