Artikelnummer: E-OSEL-H0013_48T-ES

QuicKey Pro Human POSTN/OSF-2 (Periostin) ELISA Kit, 48T

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human POSTN/OSF-2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human POSTN/OSF-2 are added to the micro ELISA plate wells. Human POSTN/OSF-2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human POSTN/OSF-2.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human POSTN/OSF-2. The concentration of Human POSTN/OSF-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Produktspezifikation

Grundlegende Informationen

Marken: Elabscience

Versand: ice bag

Speicher: 2-8℃

Haltbarkeit: 6 months

Herkunftsort: China

Technical Specifications

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Sensitivity:1.52 ng/mL

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Precision:Both intra-CV and inter-CV are < 10%.

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Shelf Life:6 months

Storage:2-8℃

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Parameterinformationen

Materialien:

Erkennungsbereich: 3.12-200 ng/mL

Testprinzip: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human POSTN/OSF-2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human POSTN/OSF-2 are added to the micro ELISA plate wells. Human POSTN/OSF-2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human POSTN/OSF-2.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human POSTN/OSF-2. The concentration of Human POSTN/OSF-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Inter-Lebenslauf:

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Testzeit: 1 h 30 min

Sensibilität: 1.52 ng/mL

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Anwendung: This ELISA kit applies to the in vitro quantitative determination of Human POSTN/OSF-2 concentrations in serum, plasma.

Spezifität:

Präzision: Both intra-CV and inter-CV are < 10%.

Quantitativ/Qualitativ:

Wiederherstellung: 80%-120%

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