8-epi-PGF2α (8-Epi-Prostaglandin F2 Alpha) ELISA Kit, 48T

Brands:Elabscience

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Place of Origin:China

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Shipping:ice bag

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Sensitivity:9.38 pg/mL

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Shelf Life:12 months

Storage:2-8℃/-20℃

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Detection Type:Colormetric

Detection Range: 15.63-1000 pg/mL

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Test Principle:This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 8-epi-PGF2α. During the reaction, Universal 8-epi-PGF2α in the sample or standard competes with a fixed amount of Universal 8-epi-PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 8-epi-PGF2α. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 8-epi-PGF2α in tested samples can be calculated by comparing the OD of the samples to the standard curve.

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Assay Time:2 h 30 min

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