Beyo3D™ Annexin V-EGFP Apoptosis Detection Kit, 50 T

Brands:Beyotime

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Place of Origin:China

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Component:Beyo3D™ Annexin V-EGFP (20X), Beyo3D™ Annexin V-EGFP Binding Buffer, Beyo3D™ PI Staining Solution (100X)

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Shelf Life:12 months

Storage:4℃/-20℃

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Em:510/617nm

Ex:488/535nm

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Detection Type:Fluorometric method

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Test Principle:Annexin V-EGFP stains both apoptotic and necrotic cells. For cells undergoing necrosis, PS inside the cell membrane will also be stained by Annexin V-EGFP due to the destroyed integrity of the cell membrane. Propidium Iodide (PI) is provided in this kit to stain necrotic cells and late apoptotic cells. Healthy cells are not stained by either Annexin V-EGFP or PI. Early apoptotic cells are Annexin V-EGFP positive and PI negative, whereas late apoptotic cells and necrotic cells are positive for both Annexin V and PI.

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Assay Time:15-30 minutes

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Detection Instrument :fluorescence microscope or microplate reader

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