BeyoCRISPR™ Quick Construction Kit (Puro), 10 T

Brands:Beyotime

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Place of Origin:China

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Component:Linearized pU6-gRNA-Cas9-T2A-Puro (15ng/μl), Ultrapure Water, 10X Quick Construction Buffer, Quick Ligase, ds Control Oligo (1μM), hU6-F Primer (10μM)

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Shelf Life:24 months

Storage:-20℃

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Test Principle:Users only need to synthesize target-specific DNA oligos, anneal to form double strands, and then ligate to the linearized vector provided in this kit for CRISPR/Cas9-based gene editing of interest. After transfection, positive mammalian cells expressing Cas9 and gRNA can be screened by Puro resistance, where gene editing usually occurs.

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