Biotin Quantitative Determination ELISA Kit, 96T

Brands:Elabscience

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Place of Origin:China

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Shipping:ice bag

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Sensitivity:0.19 ng/mL

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Precision:Both intra-CV and inter-CV are < 10%.

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Shelf Life:12 months

Storage:2-8℃

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Detection Type:Colormetric

Detection Range: 0.31-20 ng/mL

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Test Principle:This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate providedin this kit has been pre-coated with Biotin. During the reaction, Biotin in samples orStandard competes with a fixed amount of Biotin on the solid phase supporter for sites onthe Avidin-HRP specific to Biotin. Excess Avidin-HRP and unbound sample or standardare washed from the plate. Then a TMB substrate solution is added to each well. Theenzyme-substrate reaction is terminated by the addition of stop solution and the colorchange is measured spectrophotometrically at a wavelength of 450±2 nm. Theconcentration of Biotin in the samples is then determined by comparing the OD of thesamples to the standard curve.

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Assay Time:1 h 30 min

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