Calcein/PI Cell Viability/Cytotoxicity Assay Kit, 500T

Brands:Beyotime

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Shipping:Dry lce

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Component:Calcein AM (1000X), Assay Buffer, PI (1000X)

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Shelf Life:1 year at -20 ℃

Storage:-20 ℃

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Em:514nm; 617nm

Ex:494nm; 535nm

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Test Principle:Calcein AM is a non-fluorescent, hydrophobic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein, a hydrophilic and strongly fluorescent compound, which is well-retained in the cell cytoplasm and stain viable cells with strong green fluorescence. Dead cells can not be labeled with fluorescent calcein because dead cells have no or very low esterase activity. However, PI is a cell membrane impermeable dye and only stains dead cells with compromised cell membrane integrity, emitting bright red fluorescence when binding to DNA in nuclei of dead cells. Therefore, Calcein AM can be used in combination with PI for simultaneous detection of cell viability and cytotoxicity.

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Result Type:Quantitative

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Assay Time:3 min

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Detection Instrument :Flow cytometer, fluorescence microscope, or fluorescence microplate reader

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