Chromogenic LAL Endotoxin Assay Kit, 160 T

Brands:Beyotime

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Place of Origin:China

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Shipping:Blue Ice/Dry Ice

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Component:Endotoxin Standard (20EU), Assay Reagent, Assay Reagent Preparation Buffer, Chromogen, Buffer A, Buffer B, Buffer C, Endotoxin-free Water

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Sensitivity:0.1-1.0EU/ml or 0.01-0.10EU/ml

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Shelf Life:24 months

Storage:4℃

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Detection Type:Colorimetric method

Detection Range: 0.1-1.0EU/ml or 0.01-0.10EU/ml

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Test Principle:Lyophilized Amebocyte Lysate (LAL) is made from amoebocytes of Tachypleus tridentatus. It contains factor C, factor B, coagulase, and coagulinogen. Under suitable conditions, endotoxin activates C factor, followed by a series of enzymatic reactions, activating coagulinogen to form coagulase which cleaves the chromogen supplied in this kit to produce a yellow p-nitroaniline (pNA, λmax = 405nm). The amount of pNA produced is positively correlated with the endotoxin concentration over a certain period of time. Meanwhile, pNA can react with the azo reagent to produce a rose-red end product (λmax = 545nm), by which the endotoxin concentration of the sample can be determined and the interference of the sample color with the absorbance at 405 nm can be avoided as well.

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Result Type:Quantitative

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Assay Time:16 minutes

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Detection Instrument :spectrophotometer or plate reader

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