Comet Assay Kit, 100 T

Brands:Beyotime

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Place of Origin:China

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Shipping:RT/Blue Ice/Dry Ice

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Component:Lysis Buffer, DMSO, Normal Melting Point Agarose, Low Melting Point Agarose, Propidium Iodide Solution

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Shelf Life:12 months

Storage:4℃

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Em:617nm

Ex:535nm

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Test Principle:Low-melting agarose is featured by maintaining a liquid state at 37℃, which is helpful in maintaining cells in their original state without causing additional DNA damage. Therefore, the cells can be embedded in low melting agarose gel on a glass slide. After cell lysis, the migration rate of large molecule DNA from normal cells is basically the same during electrophoresis, with a very slight tailing. However, DNA single or double strand breakage generates DNA fragments of different size which migrate at different rates during electrophoresis. The more severe the DNA damage, the smaller the DNA fragments, the faster the migration rate, and the longer the comet-like tailing. Therefore, the degree of DNA damage in individual cells can be quantified by measuring the optical density or length of the comet tail portion.

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Detection Instrument :fluorescence microscope

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