GreenNuc™ Caspase-3 Assay Kit for Live Cells, 100-200T

Brands:Beyotime

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Shipping:Dry lce

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Component:GreenNuc™ Caspase-3 Substrate (1mM), Ac-DEVD-CHO(10mM)

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Shelf Life:1 year at -20 ℃

Storage:-20 ℃

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Em:530nm

Ex:500nm

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Test Principle:GreenNuc™ Caspase-3 Substrate is a fluorogenic substrate consisting of a DNA dye and a DEVD moiety specifically recognized by caspase 3/7. This negatively charged, initially non-fluorescent substrate crosses the cell membrane and enters the cytoplasm where it is cleaved by caspase3/7 in apoptotic cells to release the fluorescent DNA dye. The DNA dye migrates into the cell nucleus and binds with DNA to form the bright green fluorescent GreenNuc™-DNA complex. P

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Assay Time:15-3 min

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Detection Instrument :Flow cytometer, fluorescence microscope, or fluorescence microplate reader

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