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SKU: EK101B-48T-MS
Human IL-1β ELISA Kit-48T
This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-1β antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-1β present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1β in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).
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Product Specification
Basic Information
Brands:MULTI SCIENCES
Model Number:
Place of Origin:China
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Shipping:4℃ blue ice transportation
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Sensitivity:0.15 pg/mL
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Shelf Life:2 yrs under -4℃
Storage:4℃
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Detection Range: 3.91 pg/mL - 250 pg/mL
Research Areas:Immunology
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Test Principle:This ELISA kit uses the Sandwich-ELISA principle.
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Result Type:Quantitative
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Assay Time:3.5 h
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Detection Instrument :Microplate reader
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Form:Liquid
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