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Product Specification
Brands:Beyotime
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Place of Origin:China
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Shipping:Blue Ice/Dry Ice
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Component:Mito-Tracker Deep Red FM
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Shelf Life:24 months
Storage:-20℃
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Em:665nm
Ex:644nm
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Detection Type:Fluorometric method
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Test Principle:This product is a deep red fluorescent dye that can be passively transported across the cell membrane by simple incubation with cells and specifically labeled with biologically active mitochondria with the aid of the weakly thiol-reactive chloromethyl functional group contained in this probe, which can react with and covalently link to the sulfhydryl groups of proteins in mitochondria, so that the fluorescent labeling of mitochondria will not be lost when subsequent experiments are performed using aldehyde fixatives such as paraformaldehyde or formaldehyde, as well as the cell-permeable detergent, Triton X-100, but it may be decreased to some extent (approximately 2-8-fold). Since the excitation and emission spectra of this fluorescent probe do not usually overlap with those of common green and red fluorescent probes, it is well suited for multiple fluorescent staining experiments.
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Detection Instrument :fluorescence microscope, laser confocal microscope, flow cytometer, or fluorescence microplate reader
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