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SKU: E-BC-K803-M_96T-ES
NADP+/NADPH Colorimetric Assay Kit (WST-8), 96T
Detect total content of NADP+ and NADPH: Glucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP+ is reduced to NADPH during this reaction. NADPH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NADP+ and NADPH can be quantified by measure the OD value at 450 nm. Detect NADPH: After treating sample, heat at 60℃ water bath for 30 min. the NADP+ of the sample is decomposed and only NADPH remains. NADPH reduces WST-8 to form Formazan, and the amount of NADPH is determined by measure the OD value at 450 nm. Detect NADP+ and NADP+/NADPH: The content of NADP+ and the ratio of NADP+/NADPH in the sample can be obtained according to the total content of NADP+ and NADPH obtained of the first two steps as well as the separate content of NADPH.
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Product Specification
Basic Information
Brands:Elabscience
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Place of Origin:China
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Shipping:Ice bag
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Sensitivity:0.02 μmol/L
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Shelf Life:12 months
Storage:This product can be stored at -20°C for 12 months with shading light.
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Detection Type:Colorimetric method
Detection Range: 0.02-5.0 μmol/L
Research Areas:Oxidative Stress;Metabolic Diseases;Glycolysis and Lipid Metabolism;Cuproptosis;Disulfidptosis
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Target Synonym:NADP;NADPH
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Result Type:Quantitative
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Assay Time:70 min
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Detection Instrument :Microplate reader
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