One Step TUNEL Apoptosis Assay Kit (Green Fluorescence), 20T

Brands:Beyotime

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Shipping:Dry lce

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Component:TdT Enzyme, Fluorescein-dUTP Labeling Solution, TdT Dilution Buffer

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Shelf Life:1 year at -20 ℃

Storage:-20 ℃

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Em:570nm

Ex:550nm

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Test Principle:Fragmentation of cellular DNA is a hallmark of apoptosis. Cellular endonucleases are activated and cut genomic DNA between nucleosomes into 1-2bp DNA fragments in cells undergoing apoptosis. The One Step TUNEL Apoptosis Assay Kit employs the enzyme terminal deoxynucleotide transferase (TdT) to catalyze the incorporation of fluorescently-modified dUTP (fluorescein-dUTP) at the free 3¡¯-OH ends of fragmented DNA. This TdT-mediated dUTP Nick-End Labeling is abbreviated as TUNEL.

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Assay Time:1-2 h

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Detection Instrument :Fluorescence microscope; flow cytometer

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