QuicKey Pro Human E-Cad (E-Cadherin) ELISA Kit, 96T

Brands:Elabscience

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Place of Origin:China

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Sensitivity:46.88 pg/mL

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Precision:Both intra-CV and inter-CV are < 10%.

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Shelf Life:6 months

Storage:2-8℃

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Application:This ELISA kit applies to the in vitro quantitative determination of Human E-Cad concentrations in serum, plasma and other biological fluids.

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Detection Type:Colormetric

Detection Range: 78.13-5000 pg/mL

Research Areas:Cancer, Developmental biology, Signal transduction

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Test Principle:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human E-Cad are added to the micro ELISA plate wells. Human E-Cad in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human E-Cad.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human E-Cad. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.

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Assay Time:1 h 30 min

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