QuicKey Pro Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit, 96T

Brands:Elabscience

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Place of Origin:China

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Shipping:ice bag

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Sensitivity:0.15 ng/mL

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Precision:Both intra-CV and inter-CV are < 10%.

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Shelf Life:6 months

Storage:2-8℃

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Application:This ELISA kit applies to the in vitro quantitative determination of Human ICAM-1 concentrations in serum, plasma and other biological fluids.

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Detection Type:Colormetric

Detection Range: 0.25-16 ng/mL

Research Areas:Cancer, Cardiovascular, Immunology, Stem cells

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Test Principle:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ICAM-1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human ICAM-1 are added to the micro ELISA plate wells. Human ICAM-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human ICAM-1.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human ICAM-1. The concentration of Human ICAM-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Assay Time:1 h 30 min

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