QuicKey Pro Rat Pg (Progesterone) ELISA Kit, 48T

Brands:Elabscience

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Place of Origin:China

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Shipping:ice bag

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Sensitivity:7.01 pg/mL

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Precision:Both intra-CV and inter-CV are < 10%.

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Shelf Life:6 months

Storage:2-8℃

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Application:This ELISA kit applies to the in vitro quantitative determination of Rat Pg concentrations in serum, plasma.

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Detection Type:Colormetric

Detection Range: 15.63-1000 pg/mL

Research Areas:Signal Transduction

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Test Principle:This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat Pg are added to the micro ELISA plate wells. Rat Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

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Assay Time:1 h 30 min

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