SKU: E-CL-R0714_48T-ES

Rat PFK-A (6-phosphofructokinase Type A) CLIA Kit, 48T

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PFK-A. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PFK-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PFK-A, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat PFK-A. You can calculate the concentration of Rat PFK-A in the samples by comparing the RLU value of the samples to the standard curve.

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Product Specification

Basic Information

Brands:Elabscience

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Place of Origin:China

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Shipping:ice bag

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Sensitivity:0.19 ng/mL

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Shelf Life:6 months

Storage:2-8℃/-20℃

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Detection Type:Chemiluminescence

Detection Range: 0.31-20 ng/mL

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Test Principle:This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PFK-A. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PFK-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PFK-A, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat PFK-A. You can calculate the concentration of Rat PFK-A in the samples by comparing the RLU value of the samples to the standard curve.

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Assay Time:3 h 30 min

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