SKU: PKSH031899_100μg-ES

Recombinant Human ALK-1/ACVRL1 Protein (Fc Tag), 100 μg

Activin A receptor, type II-like 1 (ACVRL1), also known as ALK-1 (activin receptor-like kinase 1), is an endothelial-specific type I receptor of the TGF-beta (transforming growth factor beta) receptor family of ligands. On ligand binding, a heteromeric receptor complex forms consisting of two type II and two type I transmembrane serine/threonine kinases. ACVRL1 protein is expressed in certain blood vessels of kidney, spleen, heart and intestine, serving as an important role during vascular development. Mutations in ACVRL1 gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 and vascular disease.

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Product Specification

Basic Information

Brands:Elabscience

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Place of Origin:China

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Reconstitution:Please refer to the printed manual for detailed information.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

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Calculated Molecular Weight:37.4 kDa

Observed Molecular Weight:45-50 kDa

Purity: > 97 % as determined by reducing SDS-PAGE.

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Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

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Calculated Molecular Weight:37.4 kDa

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Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

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Application:Cell culture

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Bio-activity:1. Measured by its ability to bind Human ENG-Fc, latent TGFB1-His, mouse ENG-His in functional Elisa. 2. Measured by its ability to inhibit BMP9 induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953. The ED50 for this effect is typically 5-15 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.

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Formulation:Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

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