- Specification
- Downloads
- Reviews
€2.856,00
ADD TO CART
SKU: PKSM040570_100μg-ES
Recombinant Mouse DLL4 Protein (His Tag), 100 μg
Delta-like protein 4 (DLL4, Delta4), a type I membrane-bound Notch ligand, is one of five known Notch ligands in mammals and interacts predominantly with Notch 1, which has a key role in vascular development. Recent studies yield substantial insights into the role of DLL4 in angiogenesis. DLL4 is induced by vascular endothelial growth factor (VEGF) and acts downstream of VEGF as a 'brake' on VEGF-induced vessel growth, forming an autoregulatory negative feedback loop inactivating VEGF. DLL4 is downstream of VEGF signaling and its activation triggers a negative feedback that restrains the effects of VEGF. Attenuation of DLL4/Notch signaling results in chaotic vascular network with excessive branching and sprouting. DLL4 is widely distributed in tissues other than vessels including many malignancies. Furthermore, the molecule is internalized on binding its receptor and often transported to the nucleus. In pathological conditions, such as cancer, DLL4 is up-regulated strongly in the tumour vasculature. Blockade of DLL4-mediated Notch signaling strikingly increases nonproductive angiogenesis, but significantly inhibits tumor growth in preclinical mouse models. In preclinical studies, blocking of DLL4/Notch signaling is associated with a paradoxical increase in tumor vessel density, yet causes marked growth inhibition due to functionally defective vasculature. Thus, DLL4 blockade holds promise as an additional strategy for angiogenesis-based cancer therapy.
- Regular price
- €2.856,00 EUR
- Sale price
- €2.856,00 EUR
- Regular price
-
ADD TO CART
Product Specification
Basic Information
Brands:Elabscience
Model Number:
Place of Origin:China
Warranty:
Safety Performance:
Protection Class:
Sterile Status:
Reconstitution:Please refer to the printed manual for detailed information.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Battery:
Battery Service Life:
Component:
Technical Specifications
Capacity:
Concentration:
Dimension [D×W×H]:
Diameter:
Length:
Width:
High:
Volume:
Volume Range:
Speed:
Speed Range:
Max.Speed:
Max. RCF:
Temperature Range:
Operating Temperature Range:
Permissible Ambient Temperature :
Permissible Relative Humidity:
Input voltage:
Power Requirements:
Voltage/Frequency:
Sensitivity:
Specificity:
Precision:
Speed Accuracy:
Volume Accuracy:
Particle Size:
Pore Size:
Binding Capacity:
Calculated Molecular Weight:55.7 kDa
Observed Molecular Weight:68 kDa
Purity: > 96 % as determined by reducing SDS-PAGE.
Shelf Life:
Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Random Error:
Acceleration/Braking time:
Aspiration Speed:
Calculated Molecular Weight:55.7 kDa
Dilution:
Driving Motor DC motor:
Em:
Ex:
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Flow rate [liquid]:
Features and Capabilities
Application:Cell culture;ELISA
Detection Target:
Detection Type:
Detection Range:
Research Areas:
Species Reactivity:
Reactivity:
Target Synonym:
Test Principle:
Quantitative/Qualitative :
Result Type:
Materials :
Assay Time:
Autoclave component:
Available Rotor Adapters:
Binding Capacity:
Bio-activity:1. Immobilized mouse DLL4-his at 10 μg/mL (100 μL/well) can bind biotinylated mouse NOTCH1-his. The EC50 of biotinylated mouse NOTCH1-his is 30 ng/mL. 2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.2-3 µg/mL.
Bottom Shape:
Bottom Type:
Buffer:
Cap Color:
Clonality:
Conjugation:
Coupled Ligand:
Cover Type:
Detection Instrument :
Dispensing Speed:
Display:
Form:
Formulation:Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Graduation:
Growth Area: