SKU: PKSM041100_100μg-ES

Recombinant Mouse LIF Protein, 100 μg

Mouse Leukemia inhibitory factor(lif)is a secreted protein which belongs to the LIF/OSM family.LIF has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. it has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

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Product Specification

Basic Information

Brands:Elabscience

Model Number:

Place of Origin:China

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Reconstitution:Please refer to the printed manual for detailed information.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

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Calculated Molecular Weight:20.7 kDa

Observed Molecular Weight:17-25 kDa

Purity: > 95 % as determined by reducing SDS-PAGE.

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Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

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Calculated Molecular Weight:20.7 kDa

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Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

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Application:Cell culture

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Bio-activity:Measure by its ability to induce IL-6 secretion in M1 cells. The ED50 for this effect is <0.5 ng/mL. The specific activity of recombinant mouse LIF is > 2 x 106 IU/mg.

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Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

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