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SKU: PKSM041280_10μg-ES
Recombinant Mouse Osteonectin/SPARC Protein (His Tag), 10 μg
Secreted protein acidic and rich in cysteine (SPARC,BM-40) protein belongs to the family of secreted matricellular proteins with similar domain structure. Mouse SPARC protein involved an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets, and macrophages, especially in areas of tissue morphogenesis and remodeling. It appears to regulate cell growth through interactions with the extracellular matrix and cytokines. SPARC is expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair. The activity of SPARC is to modulate cell-cell and cell-matrix interactions, and its de-adhesive and growth inhibitory properties in non-transformed cells have led to studies to assess its role in cancer.
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Product Specification
Basic Information
Brands:Elabscience
Model Number:
Place of Origin:China
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Reconstitution:Please refer to the printed manual for detailed information.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
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Calculated Molecular Weight:33.6 kDa
Observed Molecular Weight:40 kDa
Purity: > 95 % as determined by reducing SDS-PAGE.
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Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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Calculated Molecular Weight:33.6 kDa
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Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
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Bio-activity:Not validated for activity
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Formulation:Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
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