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Product Specification
Brands:Beyotime
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Place of Origin:China
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Shipping:Blue Ice/Dry Ice
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Component:2X Seamless Cloning Mix, Linear pUC18 (25ng/µl), DNA fragment (1100bp, 30ng/µl), Nuclease-free water
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Shelf Life:12 months
Storage:-20℃
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Test Principle:Beyotime's Seamless Cloning Kit provides a highly efficient method for cloning DNA fragments in virtually any vectors. It employs an assembly enzyme possessing 5' exonuclease, DNA polymerase and DNA ligase activities, to fuse DNA fragments that share terminal end-homology in an in vitro cloning reaction. This kit can be used for seamlessly assembling one or more fragments and a vector simultaneously and in a precise and pre-determined order. The resulting clone is called "seamless cloning" without any additional bases.
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