T5 Exonuclease, 5000 U

Brands:Beyotime

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Place of Origin:China

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Shipping:Blue Ice/Dry Ice

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Component:T5 Exonuclease (10U/µl), 10X Reaction Buffer

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Shelf Life:36 months

Storage:-20℃

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Test Principle:Beyotime's T5 Exonuclease is a specific exonuclease that degrades double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) in the 5' to 3' direction. It is able to initiate nucleotide removal from the 5' termini or at gaps and nicks of linear or circular dsDNA. However, it does not degrade supercoiled dsDNA. T5 Exonuclease also has ssDNA endonuclease activity, which can be inhibited by reducing the Mg2+ concentration to less than 1mM in the reaction mixture. T5 Exonuclease is widely used in Gibson Assembly.

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