Science Knows No Boundaries, JET BIOFIL Supports Your Journey
As a provider of comprehensive solutions for biological laboratories, JET BIOFIL has consistently been dedicated to offering high-quality laboratory consumables to researchers. For over 20 years, our products have maintained consistent quality, ensuring that researchers can achieve optimal reproducibility and reliable results in their studies, which is why they have been widely used.
To further support the advancement of scientific research, since 2014, we have continuously organized SCI (Science Citation Index) paper collection activities. To date, we have supported the publication of over 3,000 SCI papers.
JET BIOFIL Aids in the Publication of High-Impact Papers
On January 3, 2024, Professor Huang Weihua's team from Wuhan University published a high-quality research paper titled "Nanosensor detection of reactive oxygen and nitrogen species leakage in frustrated phagocytosis of nanofibres" in Nature Nanotechnology, using JET BIOFIL’s cell culture inserts.
Article Analysis
Research Findings
Long-term exposure to inert fibrous nanomaterials (such as fiberglass or carbon nanotubes) may lead to asbestos-like pulmonary lesions, which are significant environmental and health concerns. However, the pathological mechanisms of these fibers are not yet fully understood.
The research team utilized an electrochemical nanosensor to monitor and quantify the flux and kinetics of reactive species released by individual macrophages during frustrated phagocytosis of glass nanofibers. This was done to uncover the mechanisms behind the damage to surrounding cells and lung tissue. According to their in vitro co-culture and in vivo experiments, individual macrophages, during frustrated phagocytosis, continuously release large amounts of reactive oxygen and nitrogen species. This sustained oxidative stress damages peripheral cells, eventually leading to localized chronic inflammation and lung injury.
Research Methodology
In this study, JET BIOFIL’s cell culture inserts were used for in vitro co-culture of MLE-12 cells (mouse alveolar epithelial cells) and RAW 264.7 cells (mouse monocyte-macrophage leukemia cells). This setup simulated the growth environment of peripheral cells during the phagocytosis of glass nanofibers by a single macrophage in vitro.
The experimental results showed that the reactive oxygen and nitrogen species released by RAW 264.7 during the phagocytosis of glass nanofibers damage MLE-12 cells.
Cell Culture Inserts
Cell culture inserts are commonly used in various cell experiments. They use membrane technology to simulate the original growth environment of cells, making the morphology and function of in vitro grown cells closer to those of in vivo cells.
JET BIOFIL’s cell culture inserts are made from selected polycarbonate (PC) and polyethylene terephthalate (PET) membranes, characterized by high pore density, enabling the exchange of various transmembrane substances. They are widely used in co-culture experiments, cell molecular transport, and studies involving transport, absorption, and secretion functions. The product offers a range of membrane pore sizes and specifications and also provides insertable culture dishes to meet the diverse experimental needs of customers.
